Amino acids	Catalog number	g/50 mL	Suspend in:
L-Alanine	Sigma, A7469	1.10	H2O
L-Asparagine	Amresco, 94,341	1.03	H2O
L-Aspartic Acid	Alfa Aesar, A13520	1.17	0.5 N NaOH
L-Glutamine	Amresco, 0374	1.12	H2O
L-Histidine	Amresco, 1B1164	0.65	H2O
L-Isoleucine	Amresco, E803	1.12	H2O
L-Leucine	Sigma, L8912	2.03	0.2 N HCl
L-Methionine	Amresco, E801	0.60	H2O
L-Phenylalanine	Sigma, P5482	1.01	H2O
L-Threonine	Sigma, T8441	1.11	H2O
L-Tryptophan	Amresco, E800	0.32	H2O
L-Valine	Amresco, 1B1102	1.20	H2O
L-Arginine HCl	Amresco, 0877	8.16	H2O
L-Cysteine	Sigma, 30089	1.71	1 N HCl
L-Glutamic acid	Alfa Aesar, A12919	7.59	H2O
L-Glycine	Alfa Aesar, A13816	3.84	H2O
L-Lysine HCl	Amresco, 0437	6.83	H2O
L-Proline	Sigma, P5607	4.89	H2O
L-Serine	Sigma, S4311	6.89	H2O
L-Tyrosine	Sigma, T8566	∗∗add Tyr powder
Vitamin solution	Catalog number	g/50 mL	Suspend in:
Biotin	Sigma, B4501	0.001	H2O
Ca pantothenate	Sigma, 21,210	0.039	H2O
Nicotinic acid	Sigma, N4126	0.030	H2O
Pyridoxine HCl	Sigma, P9755	0.006	H2O
Riboflavin	Sigma, R4500	0.003	H2O
Thiamine (aneurin)	Sigma, T4625	0.005	H2O
Folic acid solution	Catalog number	g/50 mL	Suspend in:
Folic acid	Sigma F8758	0.0250	0.004N NaOH
Other nutrients solution	Catalog number	g/50 mL	Suspend in:
Choline chloride	MP biomedicals, 194,639	0.3125	H2O
Myo-Inositol	Sigma, I7508	0.0315	H2O
Methyl 4-hydroxybenzoate solution	Catalog Number	g/50 mL	Suspend in:
Methyl 4-hydroxybenzoate	Sigma, H3647	5.0	95% EtOH
Buffer	Catalog Number	50 ml stock
Glacial Acetic Acid	Millipore, AX0074	1.5 mL
KH2PO4	JT Baker, 3246	1.5 g
NaHCO3	Sigma, S8875	0.5 g
Water		Up to 50 mL
Metal ions	Catalog number	g/50 mL	Suspend in:
CaCl2∗6h2o	Sigma, 21,108	12.5	H2O
CuSO4∗5h2o	Sigma, C7631	0.125	H2O
FeSO4∗7h2o	Sigma, F7002	1.25	H2O (store −20C)
MgSO4 (anhydrous)	Sigma, M7506	12.5	H2O
MnCl2∗4h2o	Sigma, M3634	0.05	H2O
ZnSO4∗7h2o	Sigma, Z0251	1.25	H2O
Cholesterol solution	Catalog Number	g/50 mL	Suspend in:
Cholesterol	Sigma, C8253	1	EtOH