Figure 2.

Effect of DHA or triacsin C on cell death in RL95-2 endometrial cancer cells. A – Cell death of cells treated with various concentrations of DHA (0–150 μM) for 24 h and assessed by flow cytometry is shown. Effect of triacsin C on cell death in RL95-2 endometrial cancer cells. B – Cell death of cells treated with various concentrations of triacsin C (0–10 μM) for 24 h and assessed by flow cytometry is shown. C – Effect of combination treatment with DHA and triacsin C on cell death in RL95-2 endometrial cancer cells. Cells were treated with 125 μM DHA, 5 μM triacsin C, or a combination of 125 μM DHA and 5 μM triacsin C for 24 h. DHA and Tc indicate docosahexaenoic acid and triacsin C, respectively. The percentage of cells with a sub-G1 DNA content was taken as a measure of cell death

At least 3 independent experiments were performed, and data shown are the mean ± standard deviation. *p < 0.05 compared to 0 μM control. ^#p < 0.001 compared with control, DHA-treated, or triacsin C-treated group.