Figure 4.

Activation of caspase-3 and -7, PARP cleavage, and XIAP in RL95-2 endometrial cancer cells by combined treatment with DHA and triacsin C. Cells were treated with 125 μM DHA, 5 μM triacsin C, or a combination of 125 μM DHA and 5 μM triacsin C for 24 h. A – Activation of caspase-3 and -7 following exposure to DHA and triacsin C, as shown by western blot analyses for caspase-3 and -7, and PARP cleavage. Equal amounts of protein (30 μg) were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotted using cleaved caspase-3, cleaved caspase-7, PARP, and XIAP antibodies, respectively. Actin was used as an internal (loading) control