Figure 9.

Suppression of apoptosis induced by combined treatment with DHA and triacsin C in the presence of caspase inhibitors in RL95-2 endometrial cancer cells. Cells were exposed to 125 μM DHA and 5 μM triacsin C for 14 h in the absence or presence of zVAD-FMK (pan caspase inhibitor), DEVE-CHO (caspase-3/-7 inhibitor), IETD-CHO (caspase-8 inhibitor), LEHD-CHO (caspase-9 inhibitor), or IETD plus LEHD, respectively. Cell death was assessed by flow cytometry. The percentage of cells with a sub-G1 DNA content was taken as a measure of cell death

At least 3 independent experiments were performed, and data shown are mean ± standard deviation. *,#p < 0.05 and p < 0.001 compared with the DHA and triacsin C-treated group, respectively. “-” – DHA and triacsin C without treatment, “+” – 125 μM DHA and 5 μM triacsin C treatment for 14 h.