Reagents: |
|
Docosahexaenoic acid (DHA) |
Sigma (St. Louis, MO, USA) |
Triacsin C (Tc) |
Sigma (St. Louis, MO, USA) |
Hoechst 33258 |
Sigma (St. Louis, MO, USA) |
Phenylmethyl-sulphonyl fluoride (PMSF) |
Sigma (St. Louis, MO, USA) |
Antibodies: |
|
Procaspase-8 |
Santa Cruz Biotech (Santa Cruz, CA, USA) |
Procaspase-9 |
Santa Cruz Biotech (Santa Cruz, CA, USA) |
ProBID |
Santa Cruz Biotech (Santa Cruz, CA, USA) |
tBID |
Santa Cruz Biotech (Santa Cruz, CA, USA) |
Cleaved caspase-8 |
Cell Signaling Tech (Beverly, MA, USA) |
Cleaved caspase-9 |
Cell Signaling Tech (Beverly, MA, USA) |
Cleaved caspase-3 |
Cell Signaling Tech (Beverly, MA, USA) |
Cleaved d caspase-7 |
Cell Signaling Tech (Beverly, MA, USA) |
Actin |
Sigma (St. Louis, MO, USA) |
PARP |
Calbiochem (San Diego, CA, USA) |
z-VAD |
Calbiochem (San Diego, CA, USA) |
DEVD-CHO |
Calbiochem (San Diego, CA, USA) |
IETD |
Calbiochem (San Diego, CA, USA) |
LEHD |
Calbiochem (San Diego, CA, USA) |
XIAP |
BD Pharmingen (San Diego, CA, USA) |