Figure 2:

Schematic workflow of antioxidant compound library screening using a 96 well plate during ischemia. Cells were isolated from 1–2 days old neonatal rat heart and seeded onto 96 well plates. Cells were incubated with antioxidant compound library and then incubated in hypoxia incubator for 24 hours. Cells were fixed and immunocytochemistry were performed with H3K27ac and actinin antibodies. Images were captured under fluorescence microscope.