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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2022 Dec 13;194:326–336. doi: 10.1016/j.freeradbiomed.2022.12.007

Figure 4: Effect of eugenol in cellular acetylation during ischemia.

Figure 4:

(A-B) The western blot shows the changes in cellular acetylation (total acetylation, H3K27ac and H3K9ac) and methylation (H3K27me3, H3K9me3) after subjecting the NRVCs to normoxia, ischemia and eugenol during ischemic conditions for a period of 24 hours. The western blot was carried out using total protein lysate and the graph displays the quantification data of the western blot (**, p value<0.05; ***, p value<0.05; ****, p value<0.0001). (C-D) Representative microscopic images show cellular acetylation during ischemia. Cells were incubated with the ischemic condition for 24 hours and fixed with 4% PFA. Immunofluorescence staining of fixed NRVCs were performed with H3K27ac (green) and actinin antibody (red). Also, the nucleus was stained with DAPI (blue). The images were observed and analyzed using the fluorescent microscope. The graph shows the quantification data of the stained NRVCs under normoxic, ischemic conditions and in the presence of eugenol under ischemic conditions (***, p value<0.05; ****, p value<0.0001).