Quantification of Zn2+ in bacterial cell extracts.
Sample | Saturation contrast | [ZS]/μM | [Zn2+]labile/μM |
---|---|---|---|
Non-induced | 0 ± 0.02 | < 1.0 | N/A |
IPTG-induced | 0.08 ± 0.01 | 5.5 | 2.4 |
IPTG-induced + TPEN | 0.05 ± 0.01 | 5.0 | 1.6 |
IPTG-induced + zinc pyrithione | 0.14 ± 0.01 | 3.4 | 7.1 |