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. 2023 Mar 13;14(14):3809–3815. doi: 10.1039/d3sc00437f

Quantification of Zn2+ in bacterial cell extracts.

Sample Saturation contrast [ZS]/μM [Zn2+]labile/μM
Non-induced 0 ± 0.02 < 1.0 N/A
IPTG-induced 0.08 ± 0.01 5.5 2.4
IPTG-induced + TPEN 0.05 ± 0.01 5.0 1.6
IPTG-induced + zinc pyrithione 0.14 ± 0.01 3.4 7.1