| Time (min) | % B | Flow Rate (nL/min) |
|---|---|---|
| 0 | l | 750 |
| 3 | l | 750 |
| 15 | 5 | 750 |
| l5.l | 5 | 300 |
| l00 | 20 | 300 |
| l23 | 45 | 300 |
| l30 | 95 | 300 |
| l35 | 95 | 300 |
| l35.l | l | 300 |
| 150 | 1 | 300 |
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