Redirection of carbon metabolism in B. licheniformis to obtain efficient alanine producer
(A) Alanine synthesis pathway and main by-product pathways in B. licheniformis. The overexpressed enzymes were marked in green, while the knockout enzymes were marked in blue.
(B) Fed-batch fermentation of B. licheniformis BLA1.
(C) The final titers and yields of fed-batch fermentations of B. licheniformis overexpressing alanine dehydrogenases from different sources.
(D) Fed-batch fermentation of B. licheniformis BLA2.