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Identification of the alternate d-alanine synthetic pathway involving d-amino acid aminotransferase Dat1
(A–C) Characterization of the d-alanine dependence on B. licheniformis BLA3Δdat1, BLA3-E1Δdat1, and BLA3-Pdat1 by testing the growth performance in media containing various concentrations of d-alanine. Triplicate experiments were carried out for physiological measurements, and error bars represent standard deviations.
(D) Fed-batch fermentation of B. licheniformis BLA3-Pdat1.
