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Effect of calcitriol on lipid deposition in HepG2 cells. Cytotoxic effects after (A) calcitriol (0-200nM) for 24 h were evaluated by CCK8 assay. (B) Intracellular TG and (C) TC were assayed in HepG2. (D) Intracellular lipid accumulation was detected by Oil red O staining method using an inverted microscope (400× magnification). Data were shown as the mean ± SEM. *P < 0.05, ***P < 0.001.
