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MK886 restrain the effect of VD treatment. (A) The cytotoxic effect after MK886 (0-100μM) for 0-2h was evaluated by CCK8 assay. (B) The best inhibiting effect after MK886 (0-10μM) for 0-2h was determined by western blot. (C, D) Effect of VD and MK886 on the protein expression involved in fatty acid uptake and β-oxidation, including PPARα, CPT1A, FABP1, CD36. The band intensity ratios were analyzed by ImageJ. Data are shown as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
