Table 2.
Electrochemical response characteristics and validation parameters of the proposed Midazolam sensors
| Parameter | PTFANI-free sensor | PTFANI-sensor |
|---|---|---|
| Slope (mV/decade)a | 53.5 | 54.5 |
| Intercept (mV)a | 301.0 | 332.4 |
| Correlation coefficient (r) | 0.9992 | 0.9998 |
| Response time (s) | 25 | 10 |
| Working pH range | 2.5–4 | 2.5–4 |
| Concentration range (M) | 1 × 10–6–1 × 10–2 | 1 × 10–6–1 × 10–2 |
| Stability (days) | 20 | 30 |
| Accuracy (mean ± SD) | 100.13 ± 1.57 | 99.89 ± 1.13 |
| Precision (% RSD) | ||
| Intra-day precisiona | 0.864 | 1.311 |
| Interday precisionb | 1.296 | 1.967 |
| LOD (M)c | 9.0 × 10–7 | 9.0 × 10–7 |
aThree different concentrations of three replicate each (n = 9) repeated three times within the same day
bThree different concentrations of three replicate each (n = 9) repeated on three successive days
cLimit of detection (measured by intersection of the extrapolated arms of non-responsive and the Nernstian segments of the calibration plot