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. 2023 Apr 5;54:33. doi: 10.1186/s13567-023-01160-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Presence of haemolysis-associated genes within the genus of Actinobacillus sensu stricto. An ML tree (1000 ultrafast bootstraps) highlighting phenotypic (orange) haemolysis of new Actinobacillus strains in relation to haemolysis-associated genes embedded within all Actinobacillus sensu stricto genomes, including RTX protein hits across the Pasteurellaceae family. Colour code (yellow-magenta) represents amino acid identity of the identified proteins as compared to the NCBI RTX protein hits. Only hits with amino acid similarity above 60% and 80% coverage are shown, and RTX-like proteins were excluded. A complete overview can be found in Additional file 4.