Fig. 1.

Effect of salt on the luminescence Gaussia luciferase. (a) In the luminescence assays, 5 μM of CTZ, containing a salt to be tested, was added to the sample containing 0.5 μM of GLuc in 50 mM MOPS buffer, pH 7.9, at 15 °C. Salt concentration ranged from 20 to 1000 mM. Error bars indicate the standard deviation. The light intensity was measured at 473 nm. (b) Effect of halogen ions on the luminescence spectra. The solution conditions are the same as in (a) but with a fixed salt concentration of 50 mM.