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Inhibition assay. Relationship between the concentration of Aza-CTZ and the maximum intensity of luminescence. For inhibition assay, 40 μL of CTZ mixture containing 300 μM ∼ 1 μM or 0 M of Aza-CTZ was added to 10 μL of GLuc solution and pipetted five times. Then the spectrum was measured. The final concentration of CTZ and GLuc was 0.5 μM and 0.2 μM, respectively.
