Table 1.
Summary of sequencing platforms introduced in this mini-review, sorted by year of development.
| Technique | Initial Year Released | Run-time | Maximum Read-length (bp) | Maximum reads/run | Approx. Error Rate | Cost per bp (JPY) |
|---|---|---|---|---|---|---|
| Sanger Sequencing | 1977 | ∼5 h | 1000 | 1 | 0.0001 | 0.06 |
| Roche 454 | 2005 | 10–48 h | 700 | 1 million | 0.01 | 0.007 |
| Illumina Genome Analyzer | 2006 | 2–10 days | 75 (paired) | 168 million | 0.01 | 0.0002 |
| Life Technologies SOLiD | 2006 | 1–2 weeks | 85 | 1 billion | 0.0001 | 0.00001 |
| Illumina HiSeq | 2009 | 1–10 days | 150 (paired) | 5 billion | 0.008 | 0.00001 |
| Illumina MiSeq | 2011 | 27 h | 300 (paired) | 300 million | 0.008 | 0.00001 |
| Oxford Nanopore Technologies MinION | 2014 | 2–3 days | 5000 | 60,000 | 0.34 | 0.0001 |