Fig. 3.

ERK activation in hypothalamic explants treated with PK2 (100 nM) and PK2β (100 nM). Representative Western Blot analysis shows p-ERK and ERK protein. Bar graphs show densitometric analysis of p-ERK/ERK protein ratio expressed as a percentage of controls. Data are expressed as mean ± SEM of three separate experiments (n = 3 per group). Statistical analyses were performed using One-way ANOVA followed by Tukey's post test where **p < 0.01 vs CTRL.