Fig. 4.

STAT3 and ERK activation in adipocytes from WT and PKR1-KO mice. Representative western blot analysis showing the ratio of protein levels of p-STAT3/STAT3 (A) and p-ERK/ERK (B), performed on adipose tissue collected from 40 week old mice. Bar graphs show densitometric analysis of the ratio of p-STAT3/STAT3 and p-ERK/ERK proteins expressed as percentage of controls. Real Time RT-PCR analysis of SOCS-3 mRNA levels in adipose tissue from 15- and 40 week old WT and PKR1-KO mice (C). Data are expressed as mean ± SEM of three separate experiments (n = 4 per group). Statistical analyses were performed using Student's t-test where *p< 0.05; **p < 0.01 vs WT.