Fig. 5.

Multiple sequence alignment of various bacterial Hfq CTRs. E. coli Hfq CTR aa presumably involved in DNA binding are indicated in red, in amyloid assembly in yellow and in DNA structural change in cyan (see 4.3). For GRAVY index hydrophobic uncharged residues are in blue, hydrophilic basic in red, hydrophilic acidic in purple and hydrophilic uncharged in dark green. The CTR regions are variable in length among bacteria and only those with a CTR comparable to that of E. coli Hfq are shown. In the alignment, strictly conserved R66 is indicated in hot pink; aa conserved in most Hfqs CTR are indicated in light pink. Other aa are indicated in yellow and green.