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. 2023 Mar 17;62:102677. doi: 10.1016/j.redox.2023.102677

Fig. 1.

Fig. 1

Tubastatin A is a novel ferroptosis inducer. (a) MDA-MB-231-ACSL4-WT and MDA-MB-231-ACSL4-KO cells were seeded in 96-well plates overnight. Following treatment with different inhibitor, cell death was detected by MTT assay. Δ Cell death means the death rate of MDA-MB-231-ACSL4-WT cells minus the death rate of MDA-MB-231-ACSL4-KO cells. (b) Cell death and lipid peroxidation measurement in the indicated cells treated with DMSO or Tubastatin A (Tub) for 20 h. Tub, 8 μM Tubastatin. (c–d) Cell death and lipid peroxidation measurement in the indicated cells treated with the indicated compounds for 20 h (c) or 28 h (d). MDA-MB-231, Tub, 8 μM; DFO, the ferroptosis inhibitor Deferoxamine mesylate, 10 μM; Fer-1, the ferroptosis inhibitor Ferrostatin-1, 10 μM. MCF-7, Tub, 10 μM; DFO, 10 μM; Fer-1, 10 μM. (e, f) Cell death and lipid peroxidation measurement in MDA-MB-231 cells treated with the indicated compounds for 20 h. Tub, 4 μM; Erastin, 2.5 μM; RLS3, 2.5 μM; DFO, 10 μM; Fer-1, 10 μM. (g, h) Cell death and lipid peroxidation measurement in MCF-7 cells treated with the indicated compounds for 28 h. Tub, 5 μM; Erastin, 5 μM; RLS3, 2.5 μM; DFO, 10 μM; Fer-1, 10 μM. (i, j) Cell viability measurement in MDA-MB-231 or MCF-7 cells treated with the indicated compounds for 24 h or 32 h, respectively. For MDA-MB-231, Tub, 4 μM; Erastin, 2.5 μM; RLS3, 2.5 μM. For MCF-7, Tub, 5 μM; Erastin, 5 μM; RLS3, 2.5 μM. b-h, Data are the mean ± s.d.; n = 3 biologically independent experiments. Statistical analysis was performed using an unpaired two-tailed Student's t-test.