Extended Data Fig. 3.

IR enhances GPX4 expression by increasing the transcription of GPX4. (a, b) MCF-7 cells treated with IR at the indicated intensities and times, then confocal microscopy of the cells were performed after staining with Nrf2 (green). (c, d) An immunoblot showing the expression of GPX4 and NRF2 in MDA-MB-231 (c) or MCF-7 (d) cells stable transfected with negative control (NC) or NRF2 shRNA with or without GPX4 overexpression. Data are representative of n = 3 biologically independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)