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. 2023 Mar 17;62:102677. doi: 10.1016/j.redox.2023.102677

Fig. 2.

Fig. 2

Tubastatin A induces ferroptosis independent of HDAC6. (a, g) An immunoblot showing the expression of HDAC6 in MDA-MB-231 (a) and MCF-7 (g) cells subjected to the indicated treatments. Data are representative of n = 3 biologically independent experiments. (b) Cell death measurement in the indicated MDA-MB-231 cells treated with DMSO or 6 μM Erastin or 5 μM RSL3 for 20 h. (c) Cell death measurement in the indicated MDA-MB-231 cells treated with DMSO or 8 μM Tub for 20 h. (d–f) Cell viability measurements in MDA-MB-231-NC or HDAC6 knocked-down MDA-MB-231 cells subjected to the indicated treatments for 36 h. (h) Cell death measurement in the indicated MCF-7 cells treated with DMSO or 25 μM Erastin or 8 μM RSL3 for 20 h. (i) Cell death measurement in the indicated MCF-7 cells treated with DMSO or 10 μM Tub for 20 h. (j–l) Cell viability measurements in MCF-7-NC or HDAC6 knocked-down MCF-7 cells subjected to the indicated treatments for 36 h. (m, n) Cell death measurement in MDA-MB-231 cells treated with the indicated compounds for 20 h. CAY, 8 μM CAY10603; SKLB, 8 μM SKLB-23bb. (o, p) Cell death measurement in MDA-MB-231 cells treated with the indicated compounds for 24 h. CAY, 4 μM CAY10603; Erastin, 2.5 μM; RLS3, 2.5 μM; SKLB, 4 μM SKLB-23bb. (q, r) Cell death measurement in MCF-7 cells treated with the indicated compounds for 28 h. CAY, 10 μM CAY10603; SKLB, 10 μM SKLB-23bb. (s, t) Cell death measurement in MCF-7 cells treated with the indicated compounds for 32 h. CAY, 5 μM CAY10603; Erastin, 5 μM; RLS3, 2.5 μM; SKLB, 5 μM SKLB-23bb. b, c, h, i, m-t, Data are the mean ± s.d.; n = 3 biologically independent experiments. Statistical analysis was performed using an unpaired two-tailed Student's t-test. d-f, j-l, Error bars are mean ± s.d., n = 3 independent repeats. Statistical analysis was performed using a two-way ANOVA analysis.