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. 2023 Mar 17;62:102677. doi: 10.1016/j.redox.2023.102677

Fig. 5.

Fig. 5

Nrf2 contributed to enhanced GPX4 expression and subsequently ferroptosis resistance and radioresistance in cancer cells. (a–b) The relative Nrf2 and GPX4 mRNA measurement in MDA-MB-231 cells (a) or MCF-7 cells (b) stable transfected with negative control (NC) or Nrf2 shRNA. (c) An immunoblot showing the expression of GPX4 and Nrf2 in MDA-MB-231 cells (left) or MCF-7 (right) stable transfected with negative control (NC) or Nrf2 shRNA. Data are representative of n = 3 biologically independent experiments. (d–e) Cell death and lipid peroxidation measurement in the indicated MDA-MB-231 cells (d) or MCF-7 cells (e) treated with the indicated compounds for 16 h (d) or 24 h (e). (d) Erastin, 8 μM; RLS3, 5 μM. (e) Erastin, 10 μM; RLS3, 7.5 μM. (f, g) Cell clone measurement in the indicated MDA-MB-231 cells (f) or MCF-7 cells (g) treated with the 2 Gy IR. (h) Lipid peroxidation measurement in the indicated MDA-MB-231 cells (left) or MCF-7 cells (right) treated with the 6 Gy IR. (i, k) Cell clone measurement in the indicated MDA-MB-231 cells (i) or MCF-7 cells (k) treated with the 2 Gy IR. (j, l) Lipid peroxidation measurement in the indicated MDA-MB-231 cells (j) or MCF-7 cells (l) treated with the 6 Gy IR. a-b, d-l, Data are the mean ± s.d.; n = 3 biologically independent experiments. Statistical analysis was performed using an unpaired two-tailed Student's t-test.