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. 2021 Aug 5;1:100022. doi: 10.1016/j.bbadva.2021.100022

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©2021TheAuthor(s).PublishedbyElsevierB.V.

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Fig. 1 — Purification and Characterization of SMALPs produced from whole cell pellets and isolated membranes. SMALPs produced from isolated membranes or whole-cell pellets were analyzed by both denaturing and non-denaturing polyacrylamide gel electrophoresis (PAGE). (A) MPR1/MPA1 SMALPs purified from whole cells using either anti-FLAG or Ni-NTA resin. The SMALPs were analyzed by SDS-PAGE and SMA-PAGE. (B) MPR2 SMALPs purified from isolated membranes using either anti-FLAG or Ni-NTA resin. The SMALPs were analyzed by SDS-PAGE and SMA-PAGE. Blue stars identify SMALPs containing membrane proteins of various quaternary structures or protein complex stoichiometries.