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. 2021 Aug 5;1:100022. doi: 10.1016/j.bbadva.2021.100022

Fig. 6.

Fig. 6

Demonstration of antibody binding specificity with MPR2 and MPR1/MPA1 SMALPs using target-specific antibodies and SMALPs α-FLAG capture. Total cellular SMALPs produced with cell membrane pellets from a cell line expressing MPR2-FLAG and total cell pellets from a cell line overexpressing MPR1/MPA1 were used to demonstrate the specificity of target-specific antibodies and the various SMALPs species. SMALPs were captured on the chip surface using a cell surface captured biotinylated α-FLAG antibody and the Biacore CAP chip. (A) Assay configuration for SMALPs capture and target-specific antibody binding using a direct surface capture of a biotinylated α-FLAG antibody using the Biacore CAP chip. (B) Capture levels for all flow cells using total SMALPs from an overexpressing MPR2 -FLAG cell line and the parental cell lines as a background control. (C) Dose-dependent binding response levels of α-MPR2 antibody to α-FLAG captured MPR2 and MPR1/MPA1 SMALPs. (D) Dose-dependent binding response levels of α-MPR1 antibody to α-FLAG captured MPR2 and MPR1/MPA1 SMALPs.