Figure 6: Stimulation of macrophages with LPS leads to consistent modeling results.
A. Schematic of SDTF activation in response to TNF or LPS. TNF stimulation results in NFκB activity, while LPS stimulation results in both NFκB and IRF activity. B. Top: Experimental and simulated nucleosome counts after LPS stimulation, for NFκB-associated nucleosome locations after 0hrs and 4hrs (n=1 for each timepoint). Bottom: Analogous counts for IRF-associated nucleosome locations (n=1 for each timepoint). C. Eviction Probability Profiles associated with LPS-induced NFκB activity, using the same model parameters as the TNF-induced data (green) and direct fit (blue). D. Eviction Probability Profiles associated with LPS-induced IRF3 activity. *: All the parameters are the same as the fitted parameters with the TNF data (Figure 4) except for the SDTF unbinding fraction, BF. (See also Figure S5).