Fig. 3.

Differential regulation of accessory cell-associated determinants following TSA treatment

The expression levels of genes associated with accessory cells were analyzed in (a) the spleen and (b) the pancreas. Gene expression was analyzed by qRT-PCR using the same cDNA preparations as shown in Fig. 2. Data indicate arithmetic mean +/- SD of three determinations. Representative data from three independent experiments are depicted (n=5 mice per group/experiment). Asterisks indicate statistical significance in the spleen samples from prediabetic, diabetic, and TSA-treated mice, as assessed by two-way ANOVA (a) and by two-tailed unpaired t-test between diabetic and TSA-treated pancreas (b) (P<0.05). Increased values between prediabetic and diabetic mice were indicated by double asterisks, whereas the decrease in values in TSA-treated mice compared to diabetic mice was denoted by a single asterisk.