Fig. 5.

RUNX1 transactivated the C11orf21 promoter through binding to the RUNX1-binding site.

(A) pGL3-C11orf21 was transfected into K562 cells with control plasmid or RUNX1, RUNX1 R174Q-mutant, or RUNX1-ETO, with or without CBFβ expression plasmids. The result of the luciferase assay is shown as a bar graph. R174Q and RUNX1-ETO reduced C11orf21 promoter activity regardless of CBFβ status.

(B) pGL3-C11orf21 and pGL3-C11orf21-mt were transfected into K562 cells. The result of the luciferase assay is shown as a bar graph. When the RUNX1-binding site was mutated (-mt), the promoter activity of pGL3-C11orf21 was attenuated. (C) pGL3-C11orf21 and pGL3-C11orf21-mt were transfected into K562 cells with control plasmid or RUNX1 in the presence of the CBFβ expression vector. The results of the luciferase assay are shown as a bar graph. Mutation of the RUNX1-binding site reduced the response for RUNX1. The data represent the mean of triplicate determinations +/- S.D.