Fig. 6.

RUNX1 transactivation of C11orf21 was suppressed by RUNX1-ETO.

(A) RUNX1-ETO suppressed RUNX1 transactivation of C11orf21 in a dose-dependent manner. Fixed doses of pGL3-C11orf21, pRc/CMV-RUNX1 and pRc/CMV-CBFβ were co-transfected into K562 cells with increasing doses of RUNX1-ETO. (B) pGL3-C11orf21 was transfected into Kasumi-1 cells with or without RUNX1 and CBFβ expression plasmids. RUNX1 expression restored the transcription activity of the C11orf21 promoter. (C) RUNX2, RUNX3, and RUNX1 were competent to activate the C11orf21 promoter in the luciferase assay experiments. The data are the mean +/- S.D (n = 3). (D) Chromatin immunoprecipitation (ChIP) data. RUNX1, RUNX2, RUNX3, and ETO binding peaks detected on the C11orf21 promoter region are shown. Data were reanalyzed by ChIP-Atlas. The structure of the C11orf21 gene is shown below the histograms of ChIP data. Black boxes indicate exons. Black arrow shows the transcription start site and red arrows show RUNX1-binding sites with TGTGGT sequences.