Figure 6.

Cell compatibility and controlled release of the let-7a antagomir.

(A) Cell compatibility was detected by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay with 100 μL mixture culture medium (24 and 48 hours Hydrogel group) or complete medium (control group, Con) for 48 hours at the absorbance of 490 nm (n = 3). (B) Cell viability was detected by Cell Counting Kit-8 (CCK8) assay in the 24 and 48 hours Hydrogel groups and control group 2 hours after CCK-8 treatment by measuring optical density at 450 nm (n = 3). (C) Fluorescence detection of Cy3-labeled let-7a antagomir at 4 weeks after surgery in nerves bridged with let-7a antagomir (anti-let-7a) or chitosan-hydrogel scaffold containing non-targeting antagomir control (Con). Boxed areas in the left images were enlarged and shown on the right. Data are presented as the mean ± SEM (n = 3).