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. 2022 Oct 11;18(7):1591–1600. doi: 10.4103/1673-5374.355979

Figure 8.

Figure 8

miR-138-5p inhibits SC migration by targeting PLLP.

(A) The number of migrating SCs in the miR-138-5p group was less than that in the mimic-NC group via the Transwell assays. Further, the number of migrating SCs in the miR-138-5p-inh group was more than that in the inh-NC group. Scale bars: 100 μm. (B) Quantification of the number of migrating cells in A. *P < 0.05, ***P < 0.001, vs. mimic-NC or inh-NC group (independent-sample t-test). (C) The potential binding site of miR-138-5p on the 3’UTR of PLLP mRNA. The mutant sequences are marked in red. (D) Dual-luciferase reporter assays of HEK293T cells cotransfected with miR-138-5p mimics, PLLP wild-type (PLLP-wt), or PLLP-mutant type (PLLP-mut) plasmids. Y-axis: Relative luciferase activity compared with the miR-NC + PLLP-Wt group. ***P < 0.001, vs. miR-NC group (one-way analysis of variance and Tukey’s post hoc test). (E) We tested PLLP expression via western blotting in the SCs transfected with miR-138-5p mimics or miR-138-5p inhibitor. All bar graphs represent the average of three independent replicates, and the error bars are the SD. inh: Inhibitor; mut: mutant type; NC: normal control; PLLP: plasmolipin; wt: wild-type.