Skip to main content
. 2022 Oct 11;18(7):1591–1600. doi: 10.4103/1673-5374.355979

Figure 9.

Figure 9

miR-138-5p reverses the circ_0002538-mediated promotion of SCs.

(A) Transwell analysis revealed that circ_0002538 promoted SC migration, but its effect was partially neutralized by the overexpression of miR-138-5p. Scale bars: 100 μm. (B) Quantification of the number of migrating cells in (A). All bar graphs represent the average of three independent replicates, and error bars are the SD. ***P < 0.001, vs. mimic-NC + vector group; ##P < 0.01, ###P < 0.001, vs. miR-138-5p + vector group; &&&P < 0.001, vs. mimic-NC + circ_0002538 group (one-way analysis of variance and Tukey’s post hoc test). (C) Western blot analyses showed that the overexpression of circ_0002538 increased PLLP protein expression, while the ectopic expression of miR-138-5p could partially eliminate this effect. (D) Quantification of PLLP by the densitometry of protein bands. *P < 0.05, vs. mimic-NC + vector group; #P < 0.05, ##P < 0.01, vs. miR-138-5p + vector group; &P < 0.05, vs. mimic-NC + circ_0002538 group (one-way analysis of variance and Tukey’s post hoc test). PLLP: Plasmolipin; NC: normal control.