Skip to main content
. 2023 Mar 24;19(3):e1011249. doi: 10.1371/journal.ppat.1011249

Fig 3. Evaluation of bivalent versus single component PmSLP vaccines using a murine infection model.

Fig 3

Survival of vaccinated and control animals after challenge with the A. BRD-PmSLP antigen-matched strain H246 or B. HS-matched H229 strain. N = 4 per group. Log-rank (Mantel Cox) tests performed for survival curve comparisons; **, p<0.01. C, D. Cumulative clinical scores depicted for individual animals over the course of 48 hours. Dotted line at 10 indicate the clinical cut-off used for determining humane endpoint. E, F. Bacterial burden in tail bleeds depicted for individual animals over time. G, H. Serum IgG titre in vaccinated or adjuvant control animals measured using purified BRD-PmSLP or HS-PmSLP proteins as capture. Boxplot with individual animals is shown. Ordinary 1-way ANOVA with Tukey’s multiple comparisons test performed on log2 transformed data to calculate p-value. ***, p<0.005; ****, p<0.0001; ns, not significant.