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. 2023 Mar 24;19(3):e1011249. doi: 10.1371/journal.ppat.1011249

Fig 5. Long term stability of PmSLP antigens.

Fig 5

A. Comparison of (i) thermal profile and (ii) first derivative of HS-PmSLP protein lyophilized and stored at 4°C or room temperature for 1 year. B. Survival of mice that received different HS-PmSLP vaccine preparations after challenge with the matched strain H229. Vaccine 1, HS-PmSLP antigen stored at -80°C for 1 year after purification. Vaccine 2, lyophilized HS-PmSLP stored for 1 year at 4°C. Both vaccines were formulated immediately prior to each dose administration. N = 4 mice for adjuvant control, N = 4 mice for each vaccinated group. Log-rank (Mantel Cox) tests performed for survival curve comparisons; **, p<0.01. C. Clinical scores of individual mice over 48 hours post intraperitoneal challenge. D. Bacteremia in tail vein sample at 15h post infection. Line at median, error bars depict interquartile range. Ordinary 1-way ANOVA with Dunnett’s multiple comparison test performed to compare vaccinated groups to adjuvant control. **, p<0.01. E. Serum IgG titre in vaccinated or control animals against the purified protein antigen. Boxplot with individual animals is shown. 1-way ANOVA with Tukey’s multiple comparisons test performed to compare each group to all other groups using log2 transformed data. ****, p<0.0001; ns, not significant.