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. 2023 Mar 24;19(3):e1011249. doi: 10.1371/journal.ppat.1011249

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© 2023 Islam et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — Ai, B: HS-PmSLP antigen specific IgG detected in serum of cattle from Trials 3 and 4 respectively. Aii: Cross-reactivity of serum from Trial 3 against BRD-PmSLP protein. (Left) Background subtracted absorbance readings obtained for each animal using serially diluted serum. Dotted line represents the cut-off used for calculating IgG titre. (Right) Boxplot displaying individual animals. 2-way ANOVA with Dunnett’s multiple comparisons test performed on log2 transformed data to compare post vaccination titres to baseline. * p<0.05, ** p<0.01, ****p<0.0001, ns = not significant. Vaccine 1 formulated with Montanide Gel02 + Poly(I:C), Vaccine 2 formulated with Alhydrogel. Adjuvant controls received Montanide Gel02 + Poly (I:C) only.