Fig. 6. P53 loss co-occurs with ETS fusion, and ETV4^AAA induces Nkx3.1-positive prostate cancer in the setting of p53 loss.

(A) Representative H&E (low magnification and high magnification of indicated area) and IHC of GFP (antibody recognizes both EGFP and EYFP), ETV4, Ki67, AR, and Nkx3.1 in anterior prostate of 12-month-old T2; Tp53^L/L; ETV4^AAA mice. (B) The mice number of different phenotypes are quantified. (C) Histological changes of the anterior prostate of 12-month-old Pbsn-Cre; Tp53^L/+; ETV4^AAA and Pbsn-Cre; Tp53^L/L; ETV4^AAA mice are shown by H&E. The expression of EGFP, ETV4, Ki67, AR, Nkx3.1, and SMA is analyzed with IHC staining. Scale bars, 50 μm. (D) The mice number of different phenotypes are quantified.