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Characterizations of cell migration. (A) Crystal violet staining of BMSCs that migrated to the lower chamber of the transwell plate after 24-h culture. (B) Manual random counting in 5 random fields under 100× magnification. (C) Wound-healing assay of a monolayer of BMSCs after 0, 6, and 24 h. Wound boundary was marked with a yellow dashed line. (D) Calculation of wound closure area after 6 and 24 h. *P < 0.05. ns, no significant difference.
