| strain, strain background (Escherichia coli) |
Rosetta |
Sigma |
70954–3 |
|
| strain, strain background (Escherichia coli) |
DH5α |
Fisher Scientific |
18265017 |
|
| cell line (Homo sapiens) |
HEK293 WT and UBQLN1, 2, 4 TKO cells, inducible UBQLN2 expression |
Itakura et al., 2016
|
|
|
| cell line (Homo sapiens) |
Human ESCs (H9) WT and UBQLN1, 2, or 4 sinbgle KO cells |
Harvard Medical School Cell Biology Initiative for Genome Editing and Neurodegeneration |
|
|
| antibody |
anti-UBQLN1/2 (Mouse Monoclonal M03) |
Abnova |
H00029978; AB_627374 |
WB (1:1000) |
| antibody |
anti-UBQLN4 (Rabbit Polyclonal) |
GeneTex |
GTX85267; AB_10725990 |
WB (1:1000) |
| antibody |
anti-Myc tab (Mouse Monoclonal GT002) |
Sigma |
SAB2702192 |
WB (1:2000) |
| antibody |
anti-PEG10 (Rabbit Polyclonal) |
Proteintech |
14412–1-AP; AB_10694427 |
WB (1:1000) |
| antibody |
anti-HA (Mouse Monoclonal HA-7) |
Sigma |
H3663; AB_262051 |
WB, IF (1:1000) |
| antibody |
anti-NC (Rabbit Polyclonal) |
Thermo Fisher (custom) |
n/a |
WB (1:1000) |
| antibody |
anti-tubulin (Mouse Monoclonal DM1A) |
Novus |
NB100-690; AB_521686 |
WB (1:20000) |
| antibody |
anti-MAP2 (Chicken Polyclonal 28225) |
Biolegend |
822501; AB_2564858 |
IF (1:2000) |
| antibody |
α-mouse IgG 680 (Goat Polyclonal) |
Licor |
926–68070; AB_10956588 |
WB (1:20000) |
| antibody |
α-mouse IgG 800 (Goat Polyclonal) |
Licor |
926–32210; AB_621842 |
WB (1:20000) |
| antibody |
α-rabbit IgG 680 (Goat Polyclonal) |
Licor |
926–68071; AB_10956166 |
WB (1:20000) |
| antibody |
α-rabbit IgG 800 (Goat Polyclonal) |
Licor |
926–32211; AB_621843 |
WB (1:20000) |
| antibody |
Alexa568 α-mouse (Goat Polyclonal) |
Invitrogen |
A11004; AB_2534072 |
IF (1:300) |
| antibody |
Alexa568 α-chicken (Goat Polyclonal) |
Invitrogen |
A11041; AB_2534098 |
IF (1:300) |
| antibody |
Alexa647 α-rabbit (Goat Polyclonal) |
Invitrogen |
A32733; AB_AB2633282 |
IF (1:300) |
| Transfected construct (Saccharomyces cerevisiae) |
LIFEACT (Abp140 AA1-17) |
GenBank; Riedl et al., 2008
|
n/a |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
gag-pol; PEG10 (AA1-708) |
GenBank |
NP_055883.2 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
gag-polASG;PEG10 (AA1-708)* D370A |
GenBank |
NP_055883.2 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
Gag; PEG10 (AA1-325) |
GenBank |
NP_001035242.1 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
CA; PEG10 (AA1-259) |
GenBank |
NP_001035242.1 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
NC; PEG10 (AA260-325) |
GenBank |
NP_001035242.1 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
Tag-less NC; PEG10 (AA260-325) |
GenBank |
NP_001035242.1 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
gag111; PEG10(AA1-111) |
GenBank |
NP_001035242.1 |
pCDNA3.1 |
| Transfected construct (Mus musculus) |
gag-pol; PEG10 (AA1-1006) |
GenBank |
NP_570947.2 |
pCDNA3.1 |
| Transfected construct (Mus musculus) |
gag-polASG;PEG10 (AA1-1006)* D420A |
GenBank |
NP_570947.2 |
pCDNA3.1 |
| Transfected construct (Homo sapiens) |
gag-pol Dendra2; PEG10 (AA1-708)-Dendra2 |
GenBank |
NP_055883.2 |
pDendra2 |
| Transfected construct (Homo sapiens) |
gag-polASG Dendra2; PEG10 (AA1-708)* D370A-Dendra2 |
GenBank |
NP_055883.2 |
pDendra2 |
| Transfected construct (Homo sapiens) |
ΔPPR Dendra2; PEG10 (AA1-681)-Dendra2 |
GenBank |
NP_055883.2 |
pDendra2 |
| Transfected construct (Macaca Mulatta) |
gag-pol Dendra2; PEG10 (AA1-742)-Dendra2 |
GenBank |
NP_001165893.2 |
pDendra2 |
| Transfected construct (Bos taurus) |
gag-pol Dendra2; PEG10 (AA1-788)-Dendra2 |
GenBank |
NP_001120682.1 |
pDendra2 |
| Transfected construct (Mus musculus) |
gag-pol Dendra2; PEG10 (AA1-1006)-Dendra2 |
GenBank |
NP_570947.2 |
pDendra2 |
| Transfected construct (Phascolarctos cinereus) |
gag-pol Dendra2; PEG10 (AA1-624)-Dendra2 |
GenBank |
XM_021000084.1 (+downstream CDS continuation to pol stop codon) |
pDendra2 |
| Transfected construct (Phascolarctos cinereus) |
gag-pol Dendra2+HsPPR; Pc PEG10 (AA1-624)+Hs PEG10 (AA682-708) Dendra2 |
GenBank |
XM_021000084.1 (+downstream CDS continuation to pol stop codon) And NP_055883.2
|
pDendra2 |
| Transfected construct (Homo sapiens) |
6XHIS-SUMO2-NC; 6 X His tag + Sumo2 tag+PEG10 (AA260-325) |
GenBank |
NP_001035242.1 |
pETSUMO2 |
| chemical compound, drug |
cycloheximide |
Sigma |
01810 |
|
| chemical compound, drug |
Bortezomib |
MP Biomedicals |
IC0218385905 |
|
| other |
Prolong Gold DAPI anti-fade mounting media |
Invitrogen |
P36941 |
Mounting medium for coverslips |
