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. 2023 Apr 6;12(4):259–273. doi: 10.1302/2046-3758.124.BJR-2022-0384.R1

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© 2023 Author(s) et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND 4.0) licence, which permits the copying and redistribution of the work only, and provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc-nd/4.0/

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Fig. 8 — Dihydrocaffeic acid (DHCA) attenuated mouse knee cartilage degradation in vivo. a) Radiograph, b) haematoxylin and eosin (H&E) staining, and c) Safranin O/fast green staining of the knee articular cartilage among the SHAM, DMM, and DMM + DHCA group (magnification: ×100 for 400 μm, ×200 for 200 μm). d) Osteoarthritis Research Society International (OARSI) scoring analysis demonstrated the degree of cartilage degradation (n = 8). e) Immunohistochemical (IHC) staining and f) quantitative analysis of positive chondrocytes about anabolism (aggrecan and collagen II) and catabolism (MMP13) (n = 3). Data were presented as means and standard deviations. *p < 0.05; **p < 0.01; ***p < 0.001.