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. 2023 Feb 2;85(3):371–378. doi: 10.1292/jvms.22-0410

Table 1. Sequences of specific primers and virulence identification primers.

Gene(s) Primer sequence (5′ to 3′) Size (bp) Reference
ent ent-F:AGCGCAGGCGGTTTCTTAA 678 [26]
ent-R:CTCGTTGTACTTCCCATTGT
cylA cyl-F:ACTCGGGGATTGATAGGC 688 [55]
cyl-R:GCTGCTAAAGCTGCGCTT
esp esp-F:AGATTTCATCTTTGATTCTTGG 510
esp-R:AATTGATTCTTTAGCATCTGG
asa1 asaI-F:GCACGCTATTACGAACTATGA 375
asaI-R:TAAGAAAGAACATCACCACGA
gelE gelE-F:TATGACAATGCTTTTTGGGAT 213
gelE-R:AGATGCACCCGAAATAATATA
hyl hyl-F:TATGGGTAATGCTGGTCG 220 This study
hyl-R:GTCCCTTGCTTCGTGTTT
efaAfs efaAfs-F:GACAGACCCTCACGAATA 705 [30]
efaAfs-R:AGTTCATCATGCTGTAGTA
efaAfm efaAfm-F:AACAGATCCGCATGAATA 735
efaAfm-R:CATTTCATCATCTGATAGTA
ace ace-F:CAACCGAATGTGATAGAAA 411 This study
ace-R:GTAACGGACGATAAAGGA