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. 2023 Mar 22;616(7955):168–175. doi: 10.1038/s41586-023-05838-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, qRT-PCR of Rhoj and Rhoq expression validating the specific downregulation of Rhoj after shRNA knockdown (Rhoj sh) in human breast cancer cell line MDA-MB-231 as compared to control cells. (n = 3 independent experiments, data are normalized using housekeeping gene Polr2a; Control shRNA (Control) is an empty shRNA vector). b, Relative cell number as compared to day 0 in MDA-MB-231 control and RhoJ shRNA knockdown cultured cells in vitro and quantified by performing crystal violet assay (Medians and range are shown, two-sided Mann-Whitney U-test, n represents triplicates from two biological replicates). c-d, Representative images (c) and cell survival analysis (d) after 48h of treatment with 8, 16, 24 μM cisplatin; 30, 100, 300 nM paclitaxel; 250, 500, 700 nM doxorubicin of MDA-MB-231 control cells and Rhoj shRNA knockdown (scale bar, 500 μm; box boundaries represent 25th and 75th percentiles; whiskers, minimum and maximum; centre line, median, two-sided Mann-Whitney U-test, n represents replicates from two biological replicates).