Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 29;616(7955):143–151. doi: 10.1038/s41586-023-05869-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a: UMAP (uniform manifold approximation and projection) scatterplots of donor P13 snRNA-seq data (n = 37,675 nuclei) for all trophoblast cell states coloured by (from left to right) assay, cell cycle phase of the nuclei and sample (10X library). Please note: bioinformatics analyses used cannot distinguish between G0 and G1. b: UMAP scatterplot of integrated snRNA-seq and scRNA-seq of all donors’ (n = 75,042 nuclei and cells, m = 17 donors with trophoblast present) trophoblast cell states in the maternal-fetal interface coloured by cell state. c: UMAP scatterplots of all donors’ scRNA-seq and snRNA-seq data for all donors’ (n = 75,042 nuclei and cells, m = 17 donors with trophoblast present) trophoblast cell states coloured by assay, sample (10X library), cell cycle phase of the cells/nuclei, donor and developmental age. d: Dot plot showing normalised, log-transformed and variance-scaled expression of genes (Y-axis) characteristic of trophoblast cell states (X-axis) in all donors (m = 17 donors with trophoblast present). e: Dot plot showing normalised, log-transformed and variance-scaled expression of genes (X-axis) characteristic of VCT cell states (Y-axis) in all donors (m = 17 donors with trophoblast present). f: Results of PAGA trajectory inference of all trophoblast cell states in donor P13 snRNA-seq data. (left) main manifold, center: denoised PAGA manifold, (right) PAGA reconstruction of putative trajectory tree for all trophoblast cell states. For the purpose of this analysis all EVTs have been united in annotation under the ‘EVT’ label. Syncytiotrophoblast (SCT), villous cytotrophoblast (VCT), cytotrophoblast cell column (CCC), proliferative (p), extravillous trophoblast (EVT), interstitial EVTs (iEVTs), giant cells (GC), endovascular EVT (eEVT), single-cell RNA sequencing (scRNA-seq), single-nuclei RNA sequencing (snRNA-seq).