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. 2023 Apr 5;6:371. doi: 10.1038/s42003-023-04718-0

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Agarose gel electrophoresis of PCR amplicons indicating the presence or absence of any recombinant insert following biopanning of the metagenomic phage display library against starch. a Biopanning against starch and elution with D-glucose. Results of a single screening experiment. Lane 1: 1 kbp DNA marker. Lanes 2–9: PCR amplicons of clones St-Glc1 to St-Glc9. b Biopanning against starch and elution with starch. Results of a single screening experiment. Lane 1: 1 kbp DNA marker. Lanes 2–9: PCR amplicons of clones St-St1 to St-St9. c–f AlphaFold2 predicted structural models of St-Glc2 (c), St-Glc8 (d), St-St4 (e), and St-St5 (f). g AlphaFold2 predicted structural model of St-Glc1. h Coomassie blue stained SDS-PAGE showing purified St-Glc1 protein following Ni-NTA metal ion affinity chromatography; Lane 1: Protein molecular weight marker; Lane 2: Purified recombinant St-Glc1 protein. i Concentration dependent binding of St-Glc1 to immobilized starch, amylose, and amylopectin. Individual data points plotted after background subtraction along with regression curves. Results of a single binding experiment with two technical replicates. Source data in Supplementary Data 3. j Binding of St-Glc1 to wells containing immobilized starch, amylose, amylopectin, or buffer (blank) with or without pre-incubation with starch, amylose, amylopectin, or D-glucose. Results of a single binding experiment. Means are plotted as bars and individual data points included. Error bars represent standard deviations of three technical replicates. Source data in Supplementary Data 3. k AlphaFold2 predicted structural model of St-Glc5v2. l Coomassie blue stained SDS-PAGE showing purified St-Glc5v2 protein following Ni-NTA metal ion affinity chromatography; Lane 1: Protein molecular weight marker. Lane 2: Purified recombinant St-Glc5v2 protein. m Concentration dependent binding of St-Glc5v2 to immobilized starch, amylose, and amylopectin. Individual data points plotted after background subtraction along with regression curves. Results of a single binding experiment of two technical replicates. Source data in Supplementary Data 3. n Binding of St-Glc5v2 to wells containing immobilized starch, amylose, amylopectin, or buffer (blank) with or without pre-incubation with starch, amylose, amylopectin, or D-glucose. Results of a single binding experiment. Means are plotted as bars and individual data points included. Error bars represent standard deviations of three technical replicates. Source data in Supplementary Data 3.