Table 2.
Ligand binding profile of clones identified by biopanning against various plant and bacterial polysaccharides
| Clonea | Biopanning ligand / eluant | Whether purified protein tested in binding assays | Ligands tested in binding assay | Ligands bound by protein |
|---|---|---|---|---|
| St-Glc1 | Starch / D-glucose | Yes | Starch, amylose, amylopectin (ELLA) | Starch, amylose |
| St-Glc5v2 | Starch / D-glucose | Yes | Starch, amylose, amylopectin (ELLA) | Starch, amylose |
| Lev-Lev5 | Levan / D-fructose | Yes | Levan, inulin, xylan, amylopectin, pectin, arabinogalactan, glucan, dextran, laminarin, amylose, starch (ELLA) | Levan, inulinb |
| BDG-Glc5 | β-D-Glucan / D-glucose | Noc | None | None |
| BDG-BDG6 | β-D-Glucan / β-D-Glucan | Noc | None | None |
| AG-Gal2 | Arabinogalactan / D-galactose | Noc | None | None |
| StapPG-GlcNAc6 | Staphylococcus aureus peptidoglycan / GlcNAc | Yes | Staphylococcus aureus, Methanobacterium sp., and Saccharomyces cerevisiae peptidoglycans (ELLA) | None |
| PBGal-Gal8 | PAA-β-D-Gal / D-galactose | Yes | PAA-biotin, biotin-PAA-β-D-galactose | None |
| PBGlcNAc-GlcNAc17F1 | PAA-β-D-GlcNAc / GlcNAc | Nod | None | None |
| PBGlcNAc-GlcNAc4F1 | PAA-β-D-GlcNAc / GlcNAc | Noc | None | None |
| PBGlcNAc-GlcNAc8 | PAA-β-D-GlcNAc / GlcNAc | Noc | None | None |
aOnly sequenced phage clones that were cloned in pET-28a(+) for recombinant expression are mentioned here.
bBinding observed at high protein concentration.
cRecombinant protein could not be expressed.
dRecombinant protein was expressed but could not be purified.