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. 2023 Apr 5;14:1880. doi: 10.1038/s41467-023-37601-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Confocal images of coronal sections showing LS A_2AR⁺ neurons terminals in the DMH and LHb. Three weeks after injecting rAAV2/9-hSyn-DIO-EYFP into the LS of A_2AR-Cre mice, EYFP-positive axonal fibers were found in the DMH and LHb. Scale bar: 500/200 μm. LV, lateral ventricle; MS, medial septum. b Experimental scheme showing that CTB488 was injected into DMH or LHb of A_2AR-tag mice (to reveal A_2AR expression via anti-HA staining). c Fluorescence images illustrating CTB488 (green) targeted to the DMH or LHb of A_2AR-tag mice and showing the co-location of DMH or LHb-projection neurons (green) and A_2AR (red) in LS. Scale bars: 200/50 μm. 3 V, third Ventricle; MHb, medial habenula. d Average number of LS-A_2AR⁺ neurons labeled with CTB488 from DMH or LHb (n = 3 mice/group, 6 slices/mouse). DMH-projecting LS-A_2AR⁺ neurons, n = 32 ± 3 cells (yellow overlap); LHb-projecting LS-A_2AR⁺ neurons, n = 14 ± 2 cells (yellow overlap). Source data are provided as a Source Data file.