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. 2023 Apr 5;14:1880. doi: 10.1038/s41467-023-37601-x

Fig. 7. A2AR overexpression in the lateral septum (LS) induces depression-like behaviors.

Fig. 7

a Adenovirus expressing A2AR (A2AR-Ad) and ZsGreen (ZsGreen-Ad) in the LS. Nuclei are stained with DAPI in blue. MS, medial septum; NAc, Nucleus Accumbens core. Scale bar: 1000 μm. b Representative Western blots and quantification of A2A receptor protein levels of mice injected with A2AR-Ad and ZsGreen-Ad (n = 6 mice/group, Unpaired t test, p = 0.0106, t(10) = 3.135). c–h Compared to control mice, A2AR-Ad mice displayed increased immobility in the tail suspension test (TST) (n = 8 mice/group, Unpaired t test, p = 0.0229, t(14) = 2.554) c, no change of the total distance traveled (n = 8 mice/group, Unpaired t test, p = 0.4639, t(14) = 0.7530) d or the time in the central area in the open field test (OFT) (n = 8 mice/group, Mann-Whitney test, p = 0.6454, U = 27) e and in the elevated O-maze test (n = 8 mice/group, Unpaired t test with Welch’s correction, p = 0.0784, Welch-corrected t(8.9) = 1.988) (f). A2AR-Ad mice displayed similar total liquid consumption (n = 8 mice/group, Mann–Whitney test, p = 0.7033, U = 28) (g) but a decreased consumption of sucrose compared with the control group (n = 8 mice/group, Mann-Whitney test, p = 0.0070, U = 7) (h) in the sucrose preference test (SPT). Data were shown as mean ± SEM. *p < 0.05, **p < 0.01; n.s., no significant difference. Source data are provided as a Source Data file.