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. 2023 Apr 3;14:1840. doi: 10.1038/s41467-023-37362-7

Fig. 1. Deficiency of Joubert syndrome GTPase ARL3 or ARL13B promotes DNA damage-induced senescence.

Fig. 1

a Immunofluorescent images for changes of primary cilia in IMR-90 cells after senescence-induction by irradiation. Cilia were labeled with acetylated tubulin. Scale bar, 10 μm. Three experiments were repeated independently with similar results. b Western blot showing changes in ciliary protein levels after senescence-induction in IMR-90 cells by irradiation. Three experiments were repeated independently with similar results. c–e SA-β-gal staining (n>100 cells per experiment) (c), western blot of senescence markers (d) and relative mRNA levels of SASP genes (e) in IMR-90 cells stably expressing control shRNA or shARL3 at day 10 post-irradiation. f–h SA-β-gal staining (f), western blot of senescence markers (g) and relative mRNA levels of SASP genes (h) in IMR-90 cells stably expressing control shRNA or shARL13B at day 10 post-irradiation. Scale bar, 200 μm. Results (c-h) from n=3 independent experiments were statistically analyzed and plotted as means ± SEM. Brown-Forsythe and Welch ANOVA tests was used for c and f. Two-tailed Student’s unpaired t-test was used for analysis in d and g. Two-way ANOVA followed by Bonferroni multiple-comparison analysis was used for e and h. Source data are provided as a Source Data file.