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. 2023 Apr 3;14:1840. doi: 10.1038/s41467-023-37362-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–c SA-β-gal staining (n>100 cells per experiment) (a), western blot of senescence markers (b) and relative mRNA levels of SASP genes (c) in IMR-90 cells stably expressing control shRNA or shFBF1 at day 10 post-irradiation. Scale bar, 200 μm. d, e TUNEL assay (n=30 cells per experiment) (d) and viability assay (e) in IMR-90 cells stably expressing control shRNA or shFBF1 at day 3 post-irradiation. f–h, SA-β-gal staining (f), western blot of senescence markers (g) and relative mRNA levels of SASP genes (h) in IMR-90 cells overexpressed with plasmid pCDH vector or pCDH-FBF1-Myc at day 7 after irradiation. Scale bar, 200 μm. All results from n = 3 independent experiments were statistically analyzed and plotted as means ± SEM. Brown-Forsythe and Welch ANOVA tests was used for a and f. Two-way ANOVA followed by Bonferroni multiple-comparison analysis was used for c. Two-tailed Student’s unpaired t-test was used for analysis in b, d, e and h. Source data are provided as a Source Data file.